In the serological method for tissue typing,if human cells expressing HLA-I are mixed with anti-HLA-I,complement,and trypan blue,what would indicate the cells are HLA-I?
A)Enzyme is released from neutrophils.
B)Cells are damaged by complement.
C)Trypan blue enters the cells.
D)Anti-HLA antibodies bind to HLAs on lymphocytes.
E)None of the answers are correct;the T cells are missing.

Question

Quizplus · Accepted Answer

If trypan blue enters the cells, it indicates that the cells are not HLA-I because trypan blue only enters cells that have lost their membrane integrity. HLA-I positive cells should not be damaged by complement, as complement is typically only activated when it binds to cells that do not express self antigens. The release of enzyme from neutrophils is not relevant to this particular serological method. Anti-HLA antibodies binding to HLAs on lymphocytes would be indicative of HLA typing, but this is not what is being measured in this particular test.

In the Serological Method for Tissue Typing,if Human Cells Expressing