In a direct ELISA test to screen for drugs in a patient's urine,what is the third step in the test process?
A)Substrate for the enzyme is added.
B)Enzyme-labeled antibody against the drug being tested is added.
C)The patient's urine sample is diluted.
D)Antibody against the drug being tested is added.

Question

Quizplus · Accepted Answer

In a direct ELISA test to screen for drugs in a patient's urine, the third step in the test process is to add the enzyme-labeled antibody against the drug being tested. This step allows for the detection of the drug in the urine sample by binding the drug to the antibody and allowing for the enzyme to be detected through a color change reaction when substrate is added. The patient's urine sample is typically diluted in a prior step and the antibody against the drug being tested is added in a later step.

In a Direct ELISA Test to Screen for Drugs in a Patient's