Figure 9.4
In Figure 9.4,the bacteria transformed with the recombinant plasmid and plated on media containing ampicillin and X-gal will
A)form blue,ampicillin-resistant colonies.
B)form blue,ampicillin-sensitive colonies.
C)form white,ampicillin-resistant colonies.
D)form white,ampicillin-sensitive colonies.
E)not grow.

Question

Figure 9.4 
In Figure 9.4,the bacteria transformed with the recombinant plasmid and plated on media containing ampicillin and X-gal will
A)form blue,ampicillin-resistant colonies. 
B)form blue,ampicillin-sensitive colonies. 
C)form white,ampicillin-resistant colonies. 
D)form white,ampicillin-sensitive colonies. 
E)not grow.

Quizplus · Accepted Answer

The recombinant plasmid contains an ampicillin resistance gene and the lacZ gene, which encodes for beta-galactosidase. When the bacteria are transformed with the recombinant plasmid and plated on media containing ampicillin and X-gal, only bacteria that have taken up the plasmid will be able to grow due to the presence of the ampicillin resistance gene. If the bacteria have also successfully incorporated the lacZ gene into their genome, they will be unable to metabolize X-gal and will produce white colonies, whereas those that have not incorporated the lacZ gene will produce blue colonies. Therefore, the correct answer is C, form white, ampicillin-resistant colonies.

Figure 9.4 in Figure 9.4,the Bacteria Transformed with the Recombinant Plasmid and Recombinant