As part of an experiment where absorbance values are measured using a spectrophotometer,you are taking readings of your sample every 20 minutes.The non-motile microbe you're testing has a generation time of roughly 20 minutes at an incubation temperature right around room temperature.Things start out fine,with the expected results - as time goes by at the correct incubation temperature,absorbance starts to rise as the medium starts to become more cloudy with growing microorganisms.But roughly 2 hours into the process,you notice that the absorbance levels flatten out,and then start to decrease unexpectedly.What is most likely taking place in your experiment?

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The Answer of As part of an experiment where absorbance values are measured using a spectrophotometer,you are taking readings of your sample every 20 minutes.The non-motile microbe you're testing has a generation time of roughly 20 minutes at an incubation temperature right around room temperature.Things start out fine,with the expected results - as time goes by at the correct incubation temperature,absorbance starts to rise as the medium starts to become more cloudy with growing microorganisms.But roughly 2 hours into the process,you notice that the absorbance levels flatten out,and then start to decrease unexpectedly.What is most likely taking place in your experiment?

As Part of an Experiment Where Absorbance Values Are Measured