Which step in the Gram stain is the critical step in differentiating gram-positive cells from gram-negative cells?
A)safranin
B)alcohol-acetone
C)iodine
D)crystal violet

Question

Quizplus · Accepted Answer

The alcohol-acetone step is the critical step in differentiating gram-positive cells from gram-negative cells. During this step, the alcohol-acetone decolorizes the gram-negative cells, making them appear colorless, while the gram-positive cells retain the crystal violet stain, making them appear purple. Therefore, this step is crucial in determining the gram status of bacterial cells.

Which Step in the Gram Stain Is the Critical Step