In a bacterial cross in which the donor (Hfr)is a⁺b⁺ and the recipient strain (F⁻)is a⁻b⁻,it is expected that recombinant bacteria will all be a⁺b⁺.

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In an Hfr-F- cross, the recombinant bacteria will not be a⁺b⁺. While some genes from the donor chromosome may be transferred to the recipient, the entire donor chromosome is not transferred, so the recipient remains a⁻b⁻. Therefore, the resulting recombinants will be a⁺ or b⁺ or both, but not necessarily both together.

In a Bacterial Cross in Which the Donor (Hfr)is A⁺b⁺