The overall purpose of DGGE is to
A) separate genomes that are less than 95% similar.
B) measure activity of cells that use DGGE as an energy source.
C) detect cells that utilize a specific radiolabeled substrate.
D) separate genes of the same length that differ in their denaturing profile due to sequence variation.

Question

Quizplus · Accepted Answer

DGGE (Denaturing Gradient Gel Electrophoresis) is a technique used to separate DNA fragments of the same length but differing in sequence. It uses a gradient of denaturants to separate fragments based on their denaturing properties, allowing for the detection of small sequence variations. Therefore, choice D is the best answer as it accurately describes the purpose of DGGE.

The Overall Purpose of DGGE Is to