What is the principle of the most commonly used test to detect b-lactamase production?
A)Chromogenic cephalosporin nitrocefin
B)Redox reaction
C)Hydrolysis of fluorogenic substrate
D)Reduction of acetoin

Question

Quizplus · Accepted Answer

The product consists of filter paper disks impregnated with nitrocefin.A disk is moistened with water or saline, and a loopful of organism is applied directly onto the disk.Within 10 minutes (or within 60 minutes for staphylococci), the area where the organisms were applied will turn red in the case of a b-lactamase-producing organism.No color change occurs with b-lactamase-negative organisms.

What Is the Principle of the Most Commonly Used Test