The goal of the primer annealing step is to:
A) Hybridize oligonucleotide primers to the denatured, single-stranded target DNA strands.
B) Separate the target DNA so that the solution can be electrophoresed and a Northern blot performed.
C) Cut the native DNA into small pieces with a restriction enzyme.
D) Amplify exponentially over many cycles of these three reaction steps.

Question

Quizplus · Accepted Answer

The goal of this step is to hybridize,or anneal,oligonucleotide primers to the denatured,single-stranded target DNA strands.A pair of primers is used in standard polymerase chain reaction (PCR): one for each strand of dsDNA.The 5' ends of these primers frame the amplification region of the target DNA.Thus,the 5' prime ends of the primer pair define the eventual size of the PCR product.Primer annealing temperatures usually range from about 45° C to about 65° C for about 30 seconds to 2 minutes.

The Goal of the Primer Annealing Step Is To