In the Voges-Proskauer test, you are testing for the metabolic by-products of which pathway?
A)Glucose ® formic acid ® formaldehyde
B)Glucose ® pyruvic acid ® mixed acid fermentation
C)Lactose ® glucose ® 2,3 butanediol
D)Glucose ® pyruvic acid ® acetoin ® diacetyl KOH

Question

Quizplus · Accepted Answer

In some bacteria, acids formed during fermentation can be further metabolized to 2,3-butanediol through the intermediate acetoin.After incubating the broth containing the bacteria and glucose, a-naphthol is added as a catalyst or color intensifier.Next, 40% KOH is added, and the tube is gently shaken to increase oxygenation.Under these conditions, acetoin is oxidized to diacetyl, which forms a red complex.

In the Voges-Proskauer Test, You Are Testing for the Metabolic