When using the isolation streaking technique,the most isolated forms of the organisms should be in which quadrant?
A) Fourth
B) Third
C) Second
D) First

Question

Quizplus · Accepted Answer

The specimen is applied by rolling the swab or placing a drop of liquid specimen onto a small area at the edge of the plate.The inoculating loop is sterilized and allowed to cool thoroughly before streaking the agar.The cooled loop is passed back and forth through the inoculum in the first quadrant several times.The first quadrant should be at least one quarter of the plate,and the streak lines should be close together.The plate is turned one-quarter turn,and quadrant two is streaked by passing the loop through the edge of the first quadrant a few times and then streaking the rest of the area.The plate is turned one-quarter turn again,and the loop is passed through the edge of quadrant two a few times and into the rest of the third quadrant.Finally,passing the loop over the final are of the agar streaks the fourth quadrant.

When Using the Isolation Streaking Technique,the Most Isolated Forms of the Organisms