What is the approximate final concentration of organisms in each well of a microdilution tray?
A)3 ´ 105 colony-forming units (CFU)/mL
B)1 ´ 105 CFU/mL
C)4 ´ 105 CFU/mL
D)5 ´ 105 CFU/mL

Question

Quizplus · Accepted Answer

An intermediate dilution of this inoculum suspension is prepared in water or saline, and a multipronged inoculator or other type of inoculating device is used to inoculate the wells to obtain a final concentration of approximately 5 ´ 10⁵.The actual dilution factor used for preparation of the intermediate dilution depends on the volume of inoculum delivered to each well by the inoculating device and the organism being tested.

What Is the Approximate Final Concentration of Organisms in Each