Which of the following is used to determine the relationship between the content of tumor cells and normal dividing cells?
A)The absorbance concentration of the fluorochrome
B)The DNA index
C)The S phase fraction
D)The G₂/M phase fraction

Question

Quizplus · Accepted Answer

The DNA index, which is a measure of the amount of DNA in a single cell compared to a normal diploid cell, can be used to determine whether tumor cells have gained or lost DNA. This can then be used to determine their relationship to normal dividing cells. The S phase fraction and G₂/M phase fraction are measures of the proportion of cells in a population that are actively dividing during those phases of the cell cycle, and may not necessarily provide information on the content of tumor cells compared to normal cells. The absorbance concentration of a fluorochrome may be used to quantify the amount of a specific biomolecule in a sample, but it would not necessarily be useful for determining the relationship between tumor cells and normal dividing cells.

Which of the Following Is Used to Determine the Relationship