Which of the following most correctly describes a shotgun technique for sequencing a genome?
A)genetic mapping followed immediately by sequencing
B)physical mapping followed immediately by sequencing
C)cloning large genome fragments into very large vectors, followed by sequencing
D)cloning several sizes of fragments into various size vectors, sequencing the clone and then ordering them
E)cloning the whole genome directly, from one end to the other

Question

Quizplus · Accepted Answer

The shotgun sequencing technique involves breaking the genome into fragments of varying sizes, cloning these fragments into vectors (often BACs), and then sequencing many copies of each clone. The sequences of the clones are then assembled in silico to generate the final genome sequence. This process is facilitated by overlapping sequences between the clones, which allow the assembly algorithm to align them in the correct order. Choice D describes this process accurately.

Which of the Following Most Correctly Describes a Shotgun Technique