Which is not an explanation of why site-directed mutagenesis is a more powerful tool than many other techniques for determining important residues in an enzyme?
A)It is a more specific technique than irreversible inhibition experiments.
B)It can be used on enzymes for which no specific irreversible inhibitor is known.
C)It allows for testing of the specific function of amino acid side chains in an enzyme.
D)It is especially useful for enzymes whose sequence is not known.

Question

Quizplus · Accepted Answer

Site-directed mutagenesis requires knowledge of the enzyme's sequence in order to design specific mutations, so it cannot be used on enzymes whose sequence is not known. Therefore, choice D is the answer. Choices A, B, and C all provide valid explanations for why site-directed mutagenesis is a more powerful tool than other techniques for determining important residues in an enzyme.

Which Is Not an Explanation of Why Site-Directed Mutagenesis Is