The Tm of a protein is temperature at which it
A)is completely denatured.
B)liquifies.
C)is half denatured.
D)resists denaturation.

Question

Quizplus · Accepted Answer

Tm (melting temperature) is the temperature at which 50% of a protein is denatured or unfolded. This can be determined by monitoring changes in absorbance or fluorescence as the temperature is increased. Option A is incorrect because at the melting temperature, only 50% of the protein is denatured, while at higher temperatures, the protein is completely denatured. Option B is incorrect as the protein does not liquify at the Tm, rather it undergoes a structural transition. Option D is incorrect as the Tm is not related to the ability of a protein to resist denaturation.

The Tm of a Protein Is Temperature at Which It