How does one determine a tryptic fingerprint to identify a protein?
A)Enzymatic treatment followed by mass spectrometry.
B)Enzymatic treatment followed by gel electrophoresis.
C)Treatment with dithiothreitol followed by isoelectric focusing.
D)Treatment with PITC followed by mass spectrometry.

Question

Quizplus · Accepted Answer

Tryptic digestion is a process where the protein sample is treated with the enzyme trypsin, which cleaves the protein into smaller peptides. These peptides can then be analyzed by mass spectrometry to identify the protein based on its unique tryptic fingerprint. This technique is known as tryptic mapping and can be a powerful tool for protein identification. Options B and C involve different treatments that may not be as reliable for identifying proteins, and option D involves a different chemical treatment that may not be as effective as tryptic digestion with trypsin.

How Does One Determine a Tryptic Fingerprint to Identify a Protein