Which enzyme would be least useful for recombination experiments that introduce new fragments of DNA into an existing DNA molecule?
A)EcoRI G↓AATTC.
B)SmaI CCC↓GGG.
C)XhoI C↓TCGAG.
D)All are equally useful.

Question

Quizplus · Accepted Answer

SmaI generates blunt ends, which are less useful for recombination experiments compared to the sticky ends produced by EcoRI and XhoI, as sticky ends facilitate the joining of complementary DNA fragments.

Which Enzyme Would Be Least Useful for Recombination Experiments That