Using the Sanger method of DNA sequencing,results in the figure below are obtained following gel electrophoresis of the DNA fragments.

Figure 18.2
A total of 21 nucleotides has been sequenced. What is the sequence of these nucleotides?
A)5'-ACCGTTTGCAGAACGTTCCCA-3'
B)5'-ACCCACGTTAAAGACCCGTTT-3'
C)5'-AAAAACCCCCCCGGGGTTTTT-3'
D)5'-ACCCTTGCAAGACGTTTGCCA-3'
E)none of the above

Question

Using the Sanger method of DNA sequencing,results in the figure below are obtained following gel electrophoresis of the DNA fragments.
  
Figure 18.2
A total of 21 nucleotides has been sequenced. What is the sequence of these nucleotides?
A)5'-ACCGTTTGCAGAACGTTCCCA-3' 
B)5'-ACCCACGTTAAAGACCCGTTT-3' 
C)5'-AAAAACCCCCCCGGGGTTTTT-3' 
D)5'-ACCCTTGCAAGACGTTTGCCA-3' 
E)none of the above

Quizplus · Accepted Answer

Counting from the bottom of the gel, the bands correspond to the nucleotide sequence 5'-ACCCTTGCAAGACGTTTGCCA-3'.

Using the Sanger Method of DNA Sequencing,results in the Figure