How does RT-PCR differ from basic PCR?
A)Reverse temperatures are used for annealing and transcription.
B)Transcription is reversed from 5'- to 3'-ends.
C)Reverse transcriptase is used to synthesize a cDNA strand complementary to an RNA strand.
D)Reverse transcriptase is used to synthesize an RNA strand from the DNA strand.

Question

Quizplus · Accepted Answer

RT-PCR (reverse transcription polymerase chain reaction) is a type of PCR that involves reverse transcription, where reverse transcriptase is used to synthesize a complementary DNA (cDNA) strand from an RNA template. This cDNA can then be amplified using PCR with specific primers for the target gene. In basic PCR, DNA is amplified using DNA polymerase and specific primers. Therefore, the key difference between RT-PCR and basic PCR is the use of reverse transcriptase in RT-PCR. Options A, B, and D are incorrect as they do not accurately describe the difference between the two techniques.

How Does RT-PCR Differ from Basic PCR