Which of the following is NOT required to perform in vitro cloning to produce a library of eukaryotic genomic DNA?
A)DNA ligase
B)a vector such as a plasmid
C)restriction endonuclease
D)mRNA

Question

Quizplus · Accepted Answer

In vitro cloning requires the use of DNA ligase to join the vector and the genomic DNA, a vector like a plasmid to insert the cloned DNA, and restriction endonucleases to cut the DNA into fragments that can be cloned. mRNA is not needed for in vitro cloning of genomic DNA, as it is a different type of nucleic acid and it is not used to build a genomic library.

Which of the Following Is NOT Required to Perform in Vitro