When would inhibition occur if the non-hydrolyzable ATP analog β,γ-methylene-ATP were given to muscle cells?
A)during formation of the cross-bridge between actin and myosin
B)during the release of ADP and Pi
C)during the ATP binding step where actin and myosin dissociate
D)during the conformational change in the myosin head

Question

Quizplus · Accepted Answer

β,γ-methylene-ATP is a non-hydrolyzable analog of ATP, it cannot be broken down by the myosin ATPase enzymes. Therefore, it would interfere with the ATP binding step where actin and myosin dissociate, which is the point in the cycle where the myosin head releases the ADP and Pi and undergoes the conformational change that powers muscle contraction.

When Would Inhibition Occur If the Non-Hydrolyzable ATP Analog β,γ-Methylene-ATP