Where is the initial bond formation located in the covalent intermediate in the chymotrypsin-catalyzed reaction?
A)between serine and the carbonyl carbon in the peptide backbone
B)between nitrogen and the carbonyl carbon in the peptide backbone
C)between histidine and the carbonyl carbon in the peptide backbone
D)between aspartate and the carbonyl carbon in the peptide backbone

Question

Quizplus · Accepted Answer

In the chymotrypsin-catalyzed reaction, the initial bond formation is located between the hydroxyl group of serine residue in the active site of chymotrypsin and the carbonyl carbon atom of the peptide bond substrate. This forms an acyl-enzyme intermediate, which is then subjected to subsequent hydrolysis reactions. Therefore, choice A is the correct answer.

Where Is the Initial Bond Formation Located in the Covalent