Because DNAs for sequencing were chosen at random with no way of knowing which organism they came from,how could genes be identified?
A)by searching for ORFs,especially those with translation start and stop sites
B)by searching for similarity between predicted ORFs and those in existing databases
C)by searching for exons bounded by consensus donor and acceptor splice sites (those that mark exon/intron boundaries)
D)A and B only
E)all of the above

Question

Quizplus · Accepted Answer

Searching for ORFs (Open Reading Frames) with start/stop sites and comparing them with known genes in existing databases is a commonly used method to identify genes from random DNA sequences obtained through sequencing. This is because genes usually have conserved sequences in the coding regions, such as start and stop codons that can differentiate them from non-coding regions. Moreover, searching for similarity between the predicted ORFs and those in existing databases can provide additional evidence for the presence of conserved domains or sequences that are characteristic of known genes. This makes options A and B both viable choices. However, searching for exons bounded by consensus splice sites (Option C) would only work if the DNA sequences were assembled into contiguous regions that contained introns and exons, which may not be the case for randomly chosen DNA sequences. Thus, option E (all of the above) is not the best choice since option C is not applicable in this scenario.

Because DNAs for Sequencing Were Chosen at Random with No