In the current form of dideoxy DNA sequencing,the primer is unlabeled and each dideoxynucleotide is labeled with a different fluorescent marker.This method
A)eliminates the need to run sequencing reaction products on a gel.
B)allows a DNA sequence to be determined from 4 separate sequencing reactions,each run on a separate lane of a gel.
C)allows a DNA sequence to be determined from one sequencing reaction that is run on a single lane of a gel.
D)eliminates the need for sophisticated machines to read the output of each sequencing reaction.

Question

Quizplus · Accepted Answer

In dideoxy DNA sequencing, the fluorescently labeled dideoxynucleotides are incorporated into the DNA strand during a single sequencing reaction that is run on a single lane of a gel. The resulting fluorescence pattern reveals the sequence of the DNA. Therefore, choice C is the correct option. Choices A, B, and D are incorrect because they do not accurately describe the current form of dideoxy DNA sequencing.

In the Current Form of Dideoxy DNA Sequencing,the Primer Is