A convenient method for assaying enzyme activity is based on measuring the conversion between the oxidized and reduced forms of nicotinamide adenine dinucleotide (NAD/NADH).Which form of nicotinamide adenine dinucleotide has a unique wavelength of absorption, and what is the wavelength at which is unique absorption is at its maximum?

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The oxidized form of nicotinamide adenine dinucleotide is abbreviated NAD, and the reduced form is abbreviated NADH.Both NAD and NADH show significant absorbance at 260 nm.These two forms differ in their absorbance at 340 nm.NADH shows significant absorbance at 340 nm, whereas NAD does not absorb at this wavelength.

A Convenient Method for Assaying Enzyme Activity Is Based on Measuring