A graduate scientist is working with a cell culture that he has determined requires a particular nutrient at a rate faster than the cell can uptake it by simple or facilitated diffusion. How would the scientist deal with this cell culture in light of this requirement?
A) He will need to genetically engineer it to integrate additional carrier or channel proteins into the cell membranes.
B) He will need to subject it to a natural mutagen such as gamma rays to produce a mutated line with a higher passive transport capacity.
C) He will need to find a new cell line that does not have this nutrient requirement.
D) He must ensure there are adequate levels of the nutrient in his media so that the cell culture can expend energy in the form of ATP and uptake it via active transport.
E) He should add a nutrient binding compound that will chelate with the nutrient, thus facilitating its uptake.
Correct Answer:
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