The frequencies with which different codons are used vary significantly between different organisms and between proteins expressed at high or low levels within the same organism. This is referred to as:

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Codon bias refers to the phenomenon where certain codons are used more frequently than others in the genome of an organism or in specific genes. This variation in codon usage can affect gene expression levels and is influenced by factors such as the organism's evolutionary history and the availability of tRNAs.

The Frequencies with Which Different Codons Are Used Vary Significantly