The Difficulty in Using X-Ray Crystallography to Determine the 3D

The difficulty in using x-ray crystallography to determine the 3D structure of a protein relies in __________.
I. solving the diffraction data
II. transferring data
III. growing single crystals
IV. solving the phase problem by multiple isomorphous replacement
V. stabilizing the crystal

A) I and II
B) I and III
C) II, III, and V
D) III and IV
E) I, III and V