Ligases are used to
A)connect two DNA fragments to each other.
B)snip DNA into smaller molecules.
C)separate DNA molecules by size.
D)determine the exact base sequence of a DNA molecule.

Question

Quizplus · Accepted Answer

Ligases are enzymes that are used to create phosphodiester bonds between two adjacent nucleotides in DNA. This enables the joining of two DNA fragments to create a single, larger molecule. Therefore, choice A is the best answer. Snipping and separating DNA are functions of other enzymes, such as nucleases and gel electrophoresis, respectively. Determining the base sequence of DNA requires DNA sequencing techniques, which typically involve the use of polymerases and primers, not ligases.

Ligases Are Used to