For the polymerase chain reaction to occur, ____.
A) isolated DNA molecules must be primed
B) all DNA fragments must be identical
C) the DNA must remain double stranded
D) a sticky end must be available for the ligase enzyme to function
E) a large sample of DNA is needed

Question

For the polymerase chain reaction to occur, ____.
A) ​isolated DNA molecules must be primed
B) ​all DNA fragments must be identical
C) ​the DNA must remain double stranded
D) ​a sticky end must be available for the ligase enzyme to function
E) ​a large sample of DNA is needed

Quizplus · Accepted Answer

PCR requires primers to bind to the targeted DNA sequence and initiate DNA synthesis. Without primers, the reaction cannot occur.

Answer: C
 PCR requires heat to denature the DNA double helix, allowing primers to bind and initiate synthesis. However, the DNA must remain double stranded during the entire PCR process for successful amplification.

Answer: E is not a correct choice as only a small amount of DNA template is required for PCR.

Answer: B and D are not correct choices as PCR can amplify different DNA fragments and does not require ligase activity.

For the Polymerase Chain Reaction to Occur, ____