The diffraction limited resolution of a standard light microscope is determined by which equation?
A) R = 0.61λNA
B) R = 0.61NA / λ
C) R = 0.61λ / NA
D) R = 0.61 / λNA

Question

Quizplus · Accepted Answer

The diffraction-limited resolution (R) of a standard light microscope is determined by the equation R = 0.61λ / NA, where λ is the wavelength of light and NA is the numerical aperture of the microscope objective. This equation is derived from the Rayleigh criterion for resolution.

The Diffraction Limited Resolution of a Standard Light Microscope Is