A cDNA is especially useful for bacterial expression of a eukaryotic protein.

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cDNA is complementary DNA that is synthesized from mRNA using the enzyme reverse transcriptase. Since mRNA is the template for protein synthesis, cDNA contains the coding sequence for a protein. Bacterial cells do not contain the machinery necessary to process eukaryotic mRNA, such as splicing or adding a 5' cap and 3' poly(A) tail. Therefore, cDNA, which lacks introns and has a poly(A) tail, is useful for bacterial expression of a eukaryotic protein.

A CDNA Is Especially Useful for Bacterial Expression of a Eukaryotic