Most eukaryotic cells transfected with DNA using liposomes will only express the introduced genes for several days because
A) foreign DNA will be targeted by CRISPR/Cas.
B) foreign RNA will be targeted by antisense RNAs.
C) the introduced DNA will not be replicated with the cells' chromosomes.
D) miRNAs will block translation of the foreign mRNA.

Question

Quizplus · Accepted Answer

Liposomes are nonviral vectors used for gene transfer. However, they are not integrated into the host cell's genome, and the introduced DNA will not be replicated with the cells' chromosomes. Therefore, the expression of the introduced genes only lasts for several days. Option A is incorrect because CRISPR/Cas is a bacterial cellular defense mechanism and not commonly found in eukaryotic cells. Option B is incorrect because antisense RNA would not block the expression of exogenous genes introduced by liposomes. Option D is incorrect because miRNAs would only affect the translation of endogenous genes and not the exogenous ones introduced by liposomes.

Most Eukaryotic Cells Transfected with DNA Using Liposomes Will Only