The regions of the DNA where RNA polymerase binds can be identified by
A) restriction mapping.
B) PCR.
C) inhibited transcription following mutagenesis in the -35 and -10 promoter regions.
D) polymerase mapping.

Question

Quizplus · Accepted Answer

Mutagenesis in the -35 and -10 promoter regions would inhibit transcription, indicating that these regions are necessary for RNA polymerase binding. Restriction mapping, PCR, and polymerase mapping do not specifically identify regions where RNA polymerase binds.

The Regions of the DNA Where RNA Polymerase Binds Can