Bacteria are grown in ¹⁵N (heavy)medium and then transferred to ¹⁴N (light)medium and are allowed to replicate for 1 generation.The DNA is subsequently isolated and centrifuged in a CsCl₂ gradient to yield what type of gradient band(s)? A)one heavy band B)one light band C)one half-heavy (intermediate to heavy and light)band D)one heavy and one half-heavy band E)one heavy and one light band

Question

Quizplus · Accepted Answer

After one generation, the bacterial DNA will be in the process of replication and will contain a mix of heavy and light isotopes. This would result in a half-heavy band in the CsCl gradient, which is intermediate to the heavy and light bands.

Bacteria Are Grown in 15N (Heavy)medium and Then Transferred to 14N

Bacteria Are Grown in ¹⁵N (Heavy)medium and Then Transferred to ¹⁴N