The protein-coding region of a eukaryotic gene ____.
A) can be amplified directly from the genome
B) is obtained by producing a cDNA copy from the gene's transcript
C) contains introns, so an mRNA copy must be produced from the gene's transcript
D) contains exons that are removed by restriction enzymes, while the remaining introns are ligated together
E) contains introns that are removed by restriction enzymes, while the remaining exons are ligated together

Question

Quizplus · Accepted Answer

The protein-coding region of a eukaryotic gene is obtained by producing a cDNA copy from the gene's transcript. This process involves reverse transcription of mRNA, which has already had its introns removed during RNA processing, resulting in a sequence that represents only the exons or the coding region.

The Protein-Coding Region of a Eukaryotic Gene ____