A researcher is interested in studying gene X, but not gene Y, in Drosophila . What does she need to amplify only gene X from the Drosophila genome?
A) some prior knowledge of the gene X sequence so that appropriate primers can be designed
B) a heat-stable restriction endonuclease
C) restriction endonucleases that cut gene X, but not gene Y
D) a cloning vector that has multiple antibiotic resistance genes
E) a cloning vector that will ligate to gene X, but not to gene Y

Question

Quizplus · Accepted Answer

To amplify a specific gene like gene X from the Drosophila genome, prior knowledge of the gene's sequence is essential for designing specific primers that will bind to the start and end of the gene. This allows for the selective amplification of gene X through PCR (Polymerase Chain Reaction), without amplifying gene Y.

A Researcher Is Interested in Studying Gene X, but Not