The purpose of using nitrocellulose for a western blot is to
A) allow the various proteins to migrate and separate
B) bind to the primary antibody
C) allow the proteins to be concentrated onto the face of the nitrocellulose where they will be accessible to the primary antibody
D) to allow the 4-chloro-1-naphthol to react and be visible

Question

Quizplus · Accepted Answer

Nitrocellulose is used in western blotting as a membrane to which proteins transferred from the gel are bound. This immobilization allows the proteins to be accessible for probing with antibodies, facilitating their detection and analysis.

The Purpose of Using Nitrocellulose for a Western Blot Is