Important steps in sequencing pure proteins include all of these except:
A) Determining the amino acid composition
B) Determining the isoionic pH of the protein
C) Breaking the protein into smaller peptides
D) Determining the amino acids on the ends of the protein
E) Determining the amino acids on the ends of the smaller peptides

Question

Quizplus · Accepted Answer

Determining the isoionic pH of the protein is not a direct step in sequencing proteins. Sequencing involves identifying the order of amino acids, which is achieved through steps like determining amino acid composition, breaking the protein into smaller peptides, and identifying the amino acids at the ends of the protein and peptides. The isoionic pH, where the protein has no net charge, is more relevant to protein purification and characterization than to sequencing.

Important Steps in Sequencing Pure Proteins Include All of These