Which would be best to separate a protein that binds strongly to its substrate?
A) Gel filtration
B) Affinity chromatography
C) Cation exchange
D) Anion exchange
E) Cation or anion exchange

Question

Quizplus · Accepted Answer

Affinity chromatography is the best method for separating a protein that binds strongly to its substrate because it utilizes a specific interaction between the protein and a ligand that is immobilized on the chromatography matrix, allowing for highly selective separation.

Which Would Be Best to Separate a Protein That Binds