A researcher inserted DNA fragments from an organism into plasmids and introduced the modified plasmids into bacterial cells.Which of the following methods would be an effective means of identifying which clones contain a specific gene of interest?
A) Sequence the DNA of the plasmids from each isolate.
B) Use a microarray to detect a transcript of the gene.
C) Isolate clones that still have the vector's genetic marker.
D) Assay for activity of the gene product.
E) Use a northern blot to detect the mRNA of the gene.

Question

Quizplus · Accepted Answer

Sequencing the DNA of the plasmids from each isolate will directly reveal the presence of the specific gene of interest by comparing the sequences to known sequences of the gene.

A Researcher Inserted DNA Fragments from an Organism into Plasmids