The method of obtaining isolated cultures that utilizes surface area to physically dilute specimens is called
A) the pour-plate technique.
B) serial dilution.
C) the streak-plate technique.
D) transport media.
E) enrichment culturing.

Question

Quizplus · Accepted Answer

The streak-plate technique involves spreading out a bacterial specimen over the surface of an agar plate, using a loop to gradually dilute the bacteria across the surface. This allows individual bacterial cells to grow into isolated colonies, which can be picked and analyzed separately. The pour-plate technique also involves using dilution, but involves adding a diluted specimen to molten agar before it solidifies, which can lead to colonies embedded within the agar rather than on the surface. Serial dilution involves diluting a specimen multiple times in order to reduce the number of bacteria in a sample. Transport media is used to preserve samples for transport without encouraging growth. Enrichment culturing involves using specialized media to encourage the growth of specific types of bacteria.

The Method of Obtaining Isolated Cultures That Utilizes Surface Area