An epidemiologist is investigating a new disease and observes what appear to be bacteria inside tissue cells in clinical samples from victims. The scientist wants to try to isolate the bacteria in the lab. What culture conditions are most likely to be successful?
A) culturing on blood agar plates
B) inoculation of EMB plates
C) incubation in an anaerobic culture system
D) inoculation of cell cultures
E) inoculation of a minimal medium broth

Question

Quizplus · Accepted Answer

Since the bacteria are found inside tissue cells, it is likely that they are obligate intracellular bacteria that require host cells to grow. Therefore, inoculation of cell cultures would be the most appropriate culture condition to successfully isolate the bacteria in the lab.

An Epidemiologist Is Investigating a New Disease and Observes What