What role does safranin play in the Gram stain procedure?
A) primary stain
B) mordant
C) decolorizing agent
D) counterstain
E) negative stain

Question

Quizplus · Accepted Answer

Safranin is the counterstain in the Gram stain procedure, which is applied after the crystal violet and iodine mordant. The safranin stains the remaining bacterial cells, which were not stained by the crystal violet-iodine complex, allowing for visualization and differentiation of gram-negative bacteria (pink/red) from gram-positive bacteria (purple/blue).

What Role Does Safranin Play in the Gram Stain Procedure