A student isolates, purifies, and combines in a test tube a variety of molecules needed for DNA replication. When she adds some DNA to the mixture, replication occurs, but each DNA molecule consists of a normal strand paired with numerous segments of DNA a few hundred nucleotides long. What has she probably left out of the mixture?
A) DNA polymerase
B) DNA ligase
C) Okazaki fragments
D) primase

Question

Quizplus · Accepted Answer

DNA ligase is necessary for joining the Okazaki fragments on the lagging strand during DNA replication. Without it, the DNA would consist of a normal strand paired with numerous short segments, as described.

A Student Isolates, Purifies, and Combines in a Test Tube