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A piece of DNA was incubated with a restriction enzyme called Hind III. The restriction enzyme digestion produced eight different size fragments that were separated by gel electrophoresis.
Which of the following observations correctly describes characteristics of the outcomes of this process?
A) When separated by gel electrophoresis, the pattern of DNA bands specific to Hind III digestion; repeating the process using a different restriction enzyme would not produce these fragments.
B) The sequence that Hind III binds is found at places in this piece of DNA; gel electrophoresis provides the force needed to break the DNA under the DNA-enzyme complexes.
C) If an electrical current is not used during gel electrophoresis, eight separate DNA bands would be visible, but they would not be separated as much as when an electrical current is used.
D) Because restriction enzymes cut specific sequences of DNA, use of any other different restriction enzyme would result in one large piece of DNA rather than eight fragments.

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Quizplus · Accepted Answer

Restriction enzymes like Hind III recognize specific DNA sequences and cut the DNA at these sites, producing fragments of various sizes. The pattern of bands observed after gel electrophoresis is unique to the restriction enzyme used because it depends on the locations of its specific recognition sites within the DNA. Using a different restriction enzyme, which would recognize and cut at different sequences, would produce a different pattern of DNA fragments.

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