Which of the following describes the best approach to making a gene that contains introns shorter, but remaining functional, for genetic engineering purposes?
A) express in bacteria then a restriction enzyme (endonuclease) to cut the gene into shorter pieces
B) isolate the specific mRNA from the animal then use reverse transcriptase to produce cDNA
C) isolate the specific protein from the animal then use the genetic code to reconstruct DNA from the polypeptide sequence
D) using DNA ligase to put together fragments of the DNA that code for a particular polypeptide

Question

Quizplus · Accepted Answer

Isolating the specific mRNA from the animal and then using reverse transcriptase to produce cDNA is the best approach for making a gene shorter but functional for genetic engineering. This process naturally removes introns since mRNA only contains the exons (coding regions) after splicing.

Which of the Following Describes the Best Approach to Making