What information is critical to the success of polymerase chain reaction (PCR)?
A) The DNA sequence of the ends of the DNA to be amplified must be known.
B) The complete DNA sequence of the DNA to be amplified must be known.
C) The sequence of restriction-enzyme recognition sites in the DNA to be amplified must be known.
D) The sequence of restriction-enzyme recognition sites in the DNA to be amplified and in the plasmid where the amplified DNA fragment will be cloned must be known.

Question

Quizplus · Accepted Answer

For PCR to be successful, it is essential to know the DNA sequence of the ends of the DNA to be amplified. This information is used to design specific primers that flank the target DNA sequence, enabling its amplification. The complete sequence of the DNA to be amplified or the sequence of restriction-enzyme recognition sites is not necessary for PCR itself.

What Information Is Critical to the Success of Polymerase Chain