Using modern techniques of sequencing by synthesis and the shotgun approach, the sequences of entire chromosomes are determined by ________.
A) systematically sequencing DNA sequences as they occur along the length of the chromosome
B) cloning chromosome sections into large plasmid, sequencing, and then reassembling the chromosome
C) fragmenting chromosomal DNA, sequencing and using computer analysis to determine regions where sequences overlap
D) identifying gaps in previously generated genetic maps, cloning those regions into plasmids and determining their DNA sequences

Question

Quizplus · Accepted Answer

The correct approach involves fragmenting chromosomal DNA, sequencing those fragments, and then using computer analysis to align and overlap these sequences to reconstruct the entire chromosome. This method is central to the shotgun sequencing technique.

Using Modern Techniques of Sequencing by Synthesis and the Shotgun